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Anti-NR1A2 / THRB Antibody (aa37-86) IHC-plus™ LS-B1005

Note: This antibody replaces LS-C30052
See our new NR1A2 / THRB ELISA Kits
Mouse/Human/Rat NR1A2 / THRB DNA Binding ELISA Kit - LS-F811
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

NR1A2 / THRB Antibody LS-B1005 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-THRB antibody IHC of human thyroid.
Western blot
THRB antibody LS-B1005 Western blot of Fetal skeletal muscle lysate.
Western blot
Fetal Skeletal Muscle Lysate

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Rabbit Polyclonal to Human NR1A2 / THRB
Human, Monkey, Rat, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated

Details

Human NR1A2 / THRB
Rabbit
Human, Monkey, Rat, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.2 - 1 µg/ml)

Specificity and Use

NR1A2 / THRB antibody was raised against synthetic peptide located between aa37-86 of human THRB (P10828, NP_000452). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Horse (100%); Galago, Elephant, Dog, Guinea pig (85%); Mouse, Rat (78%).
LS-E12666 - Lyophilized - 100 µg - $145.00
aa37-86
Human THRB
Immunohistochemistry: LS-B1005 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1005 was determined to be 5 ug/ml. This antibody was tested in a simple peptide-based ELISA.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NR1A2 / THRB

P10828 NM_000461 NP_000452.2

THRB Antibody, C-erba beta2 Antibody, C-ERBA-2 Antibody, ERBA-BETA Antibody, GRTH Antibody, Oncogene ERBA2 Antibody, THRB2 Antibody, TR-BETA Antibody, THRB1 Antibody, NR1A2 Antibody, Thyroid hormone receptor beta Antibody, Trbeta1 Antibody, Trbeta2 Antibody, C-erba beta1 Antibody, C-ERBA-BETA Antibody, ERBA2 Antibody, PRTH Antibody, THR1 Antibody, Thyroid hormone receptor, beta Antibody, Trbeta Antibody

Thyroid hormone receptor beta (TR beta), a NR1 Thyroid Hormone-Like Receptor, has been shown to affect cholesterol metabolism, hearing, cone development in the eye, and hypothalamopituitary-thyroid axis function. Mice lacking the TR beta locus exhibit elevated thyroid hormone levels, profound hearing loss, impaired weight gain, abnormal bone development, and loss of thyroid-hormone induced activation of cholesterol 7 alpha-hydroxylase (CYP7A).

Immunohistochemistry

Immunohistochemistry
Anti-THRB antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1005 concentration 5 ug/ml.

Western blot

Western blot
THRB antibody LS-B1005 Western blot of Fetal skeletal muscle lysate.

Western blot

Western blot
Fetal Skeletal Muscle Lysate
 

Requested From: United States
Date Requested: 9/2/2015

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