Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
DAX-1 (DSS-AHC critical region on the X chromosome, gene 1) is an orphan NRO Knirps-Like nuclear receptor that is involved in adrenal gland and gonad development. DAX-1 has been shown to negatively regulate transcription of the nuclear receptor steroidogenic factor 1 (SF-1). DAX-1 is also involved in the development and differentiation of anterior pituitary cells and in steroidogenesis.
IHC of paraffin-embedded Cal27 xenograft using NR0B1 antibody at 1:100 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using DAX-1 antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate). A: HeLa S3 . 7.5% SDS PAGE. NR0B1 / DAX1 antibody diluted at 1:500
NRB1 antibody immunoprecipitates NRB1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of NRB1 protein by 2 ug of NRB1 antibody 7.5% SDS-PAGE The immunoprecipitated NRB1 protein was detected by NRB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.