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Anti-NR0B1 / DAX1 Antibody (aa96-406) LS-C185691

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.4 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NR0B1 / DAX1 Antibodies

Anti-NR0B1 / DAX1 Antibody (C-Terminus) IHC-plus™ LS-A5716
Rabbit Polyclonal to Human NR0B1 / DAX1
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-NR0B1 / DAX1 Antibody (Ligand-binding Domain) IHC-plus™ LS-A5732
Rabbit Polyclonal to Human NR0B1 / DAX1
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-NR0B1 / DAX1 Antibody (aa359-408) IHC-plus™ LS-B3480
Rabbit Polyclonal (IgG) to Human NR0B1 / DAX1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NR0B1 / DAX1
Human
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human NR0B1 / DAX1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation (1:100 - 1:500)

Specificity and Use

NR0B1 / DAX1 antibody was raised against recombinant fragment corresponding to a region within amino acids 96 and 406 of NR0B1 (SwissProt P51843).
aa96-406
Human NR0B1 / DAX1
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About NR0B1 / DAX1

P51843 NM_000475 NP_000466.2

NR0B1 Antibody, AHX Antibody, AHC Antibody, AHCH Antibody, DAX1 Antibody, DSS Antibody, DAX 1 Antibody, DAX-1 Antibody, Adrenal hypoplasia congenita Antibody, Dosage-sensitive sex reversal Antibody, GTD Antibody, HHG Antibody, Nuclear hormone receptor Antibody, NROB1 Antibody, Nuclear receptor DAX-1 Antibody, SRXY2 Antibody, Nuclear receptor DAX1 Antibody, Dax-1 receptor Antibody

DAX-1 (DSS-AHC critical region on the X chromosome, gene 1) is an orphan NRO Knirps-Like nuclear receptor that is involved in adrenal gland and gonad development. DAX-1 has been shown to negatively regulate transcription of the nuclear receptor steroidogenic factor 1 (SF-1). DAX-1 is also involved in the development and differentiation of anterior pituitary cells and in steroidogenesis.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Cal27 xenograft using NR0B1 antibody at 1:100 dilution.

Immunocytochemistry

Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using DAX-1 antibody at 1:200 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: HeLa S3 . 7.5% SDS PAGE. NR0B1 / DAX1 antibody diluted at 1:500

Immunoprecipitation

Immunoprecipitation
NRB1 antibody immunoprecipitates NRB1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of NRB1 protein by 2 ug of NRB1 antibody 7.5% SDS-PAGE The immunoprecipitated NRB1 protein was detected by NRB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Requested From: United States
Date Requested: 12/9/2016

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