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Anti-NR0B1 / DAX1 Antibody (aa359-408) IHC-plus™ LS-B3480

Note: This antibody replaces LS-C101351

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.6 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NR0B1 / DAX1 Antibodies

Anti-NR0B1 / DAX1 Antibody (C-Terminus) IHC-plus™ LS-A5716
Rabbit Polyclonal to Human NR0B1 / DAX1
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-NR0B1 / DAX1 Antibody (Ligand-binding Domain) IHC-plus™ LS-A5732
Rabbit Polyclonal to Human NR0B1 / DAX1
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-NR0B1 / DAX1 Antibody (aa359-408) IHC-plus™ LS-B3480
Rabbit Polyclonal (IgG) to Human NR0B1 / DAX1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NR0B1 / DAX1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NR0B1 / DAX1
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Bat, Hamster, Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NR0B1 / DAX1 antibody was raised against synthetic peptide located between aa359-408 of human NR0B1 (Q9BG96, NP_000466). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Lizard (100%); Galago, Hamster, Xenopus, Seabass, Salmon, Stickleback, Medaka (92%); Bat (91%); Zebrafish (85%); Catfish (81%).
LS-E11054 - Lyophilized - 100 µg - $145.00
aa359-408
Human NR0B1 / DAX-1
Immunohistochemistry: LS-B3480 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3480 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

PBS, 0.09% sodium azide, 2% sucrose
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NR0B1 / DAX1

P51843 NM_000475 NP_000466.2

NR0B1 Antibody, AHX Antibody, AHC Antibody, AHCH Antibody, DAX1 Antibody, DSS Antibody, DAX 1 Antibody, DAX-1 Antibody, Adrenal hypoplasia congenita Antibody, Dosage-sensitive sex reversal Antibody, GTD Antibody, HHG Antibody, Nuclear hormone receptor Antibody, NROB1 Antibody, Nuclear receptor DAX-1 Antibody, SRXY2 Antibody, Nuclear receptor DAX1 Antibody, Dax-1 receptor Antibody

DAX-1 (DSS-AHC critical region on the X chromosome, gene 1) is an orphan NRO Knirps-Like nuclear receptor that is involved in adrenal gland and gonad development. DAX-1 has been shown to negatively regulate transcription of the nuclear receptor steroidogenic factor 1 (SF-1). DAX-1 is also involved in the development and differentiation of anterior pituitary cells and in steroidogenesis.

Immunohistochemistry

Immunohistochemistry
Anti-DAX1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3480 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
NR0B1 / DAX-1 antibody LS-B3480 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/9/2016

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