Target
Human NPC2
Synonyms
NPC2 | EDDM1 | HE1 | Epididymal protein 1 | NP-C2 | Niemann-Pick disease, type C2
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence QAEPVQFKDCGSVD, from the internal region of the protein sequence according to NP_006423.1.
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Experiments gave bands at approx 20kDa and 23kDa in Human Testis lysates. These bands correspond to earlier findings in literature with different antibodies (Lgar et al, Mol Hum Reprod. 2006 Jul;12(7):461-8; PMID: 16772431. This protein has a calculated MW of 16.6kDa according to NP_006423.1. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee