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Anti-NOTCH3 Antibody (clone HMN3-133) LS-C188664

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $235
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NOTCH3 Antibodies

Anti-NOTCH3 Antibody (aa2306-2318) IHC-plus™ LS-B2379
Goat Polyclonal to Human NOTCH3
Human, Monkey, Mouse, Rat, Bat
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NOTCH3 Antibody (C-Terminus) LS-C83739
Rabbit Polyclonal (IgG) to Human NOTCH3
Human
IHC, Western blot
Unconjugated
Anti-NOTCH3 Antibody (aa2291-2321) LS-C100466
Rabbit Polyclonal to Human NOTCH3
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Hamster Monoclonal [clone HMN3-133] (IgG) to Mouse NOTCH3
Mouse, Rat
Flow Cytometry
Unconjugated

Details

Mouse NOTCH3
Hamster
Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Monoclonal [HMN3-133]
Unconjugated
Protein G purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

NOTCH3 antibody was raised against mouse Notch 3-Fc fusion protein.
Recognizes Notch 3, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 3 is primarily expressed by proliferating neuroepithelium and arterial smooth muscle cells, and may play a role during CNS development. Notch 3 is also present on some thymocytes subsets and Treg cells, and Notch 3 signalling plays a role in mammalian T cell lineage commitment, thymocyte development, and stem cell haematopoiesis. Studies have implicated the over-expression of Notch 3 in T-cell leukaemia. In humans, mutations in the NOTCH3 gene are responsible for the heritable vascular dementia known as CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephelopathy syndrome), predisposing to early onset stroke. Studies have implicated Notch 3 as crucial for ErbB2-negative breast cancer development, and possibly as a therapeutic target for these tumors, which at present lack effective molecular targets. Clone HMN3-133 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.

Packaging

PBS, 0.09% sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About NOTCH3

Q9UM47 NM_000435 NP_000426.2

NOTCH3 Antibody, CASIL Antibody, CADASIL Antibody, Notch 3 Antibody, Notch (Drosophila) homolog 3 Antibody, Notch homolog 3 Antibody, Notch homolog 3 (Drosophila) Antibody

NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined.

Requested From: United States
Date Requested: 12/11/2016

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