Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 22G1] (IgG1,k) to Yeast Nop2p
Immunofluorescence, Western blot
Yeast (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [22G1]
Tissue culture supernatant
Immunofluorescence (1:500 - 1:5000)
Western blot (1:10000 - 1:25000)
Specificity and Use
For western blots of yeast protein samples, use 22G1 diluted 1:10000 (cell lysates) to 1:25000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try 22G1 diluted 1:1000-1:5000. For immunofluorescence on yeast cells, use 22G1 diluted 1:500-1:5000. We routinely dilute 22G1-1:1000.
Tissue culture supernatant, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.