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Anti-NLK Antibody (aa234-527) LS-C185921

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml Unavailable

Related Products

48 NLK Antibodies

Most Popular NLK Antibodies

Anti-NLK Antibody (aa406-436) LS-C99910
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Immunohistochemistry Image
Anti-NLK Antibody (aa408-436) LS-C164504
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Anti-NLK Antibody LS-C332412
Rabbit Polyclonal (IgG) to Human NLK
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Anti-NLK Antibody LS-C341557
Rabbit Polyclonal to Human NLK
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Anti-NLK Antibody LS-C412425
Rabbit Polyclonal to Human NLK
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NLK
Human, Mouse, Rat, Bovine
ICC, Western blot
Unconjugated

Details

Human NLK
Rabbit
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

NLK antibody was raised against recombinant fragment corresponding to a region within amino acids 234 and 527 of NLK (SwissProt Q9UBE8). Percent identity by BLAST analysis: Human, Rat, Bovine (100%); Xenopus (98%).
aa234-527
Human NLK

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About NLK

Q9UBE8 NM_016231 NP_057315.3

NLK Antibody, LAK1 Antibody, Nemo-like kinase Antibody, Nmo kinase Antibody, Nemo like kinase Antibody, Protein LAK1 Antibody

Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing.

Immunocytochemistry

Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using NLK antibody at 1:100 dilution.

Western blot

Western blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. NLK antibody diluted at 1:1000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. NLK antibody. NLK antibody diluted at 1:1000.

Requested From: 
Date Requested: 4/25/2017

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