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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NKD1 Antibody (aa65‑114) LS‑C82447
NKD1 antibody LS-C82447 is an unconjugated rabbit polyclonal antibody to NKD1 from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C82447-50
50 µg
$365

Popular NKD1 Products

Immunofluorescence staining of HepG2 cells at a dilution of 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: Western blot, ELISA
NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bovine, Guinea pig
Applications: IHC, IHC - Paraffin, Western blot
NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bovine, Guinea pig
Applications: IHC, IHC - Paraffin, Western blot

Product Description

NKD1 antibody LS-C82447 is an unconjugated rabbit polyclonal antibody to NKD1 from human, mouse, rat and other species. Validated for IHC and WB.
About NKD1
Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Q969G9 Q969G9

NKD1 Antibody, Dvl-binding protein Antibody, HNkd Antibody, Naked1 Antibody, Naked-1 Antibody, HNkd1 Antibody, NKD Antibody, Naked cuticle-1 Antibody


Specifications

Target
Human NKD1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Guinea pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NKD1 antibody was raised against synthetic peptide located between aa65-114 of human NKD1 (Q969G9, NP_149110). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Guinea pig (100%); Dog, Bat, Opossum (92%); Marmoset, Elephant, Turkey, Zebra finch, Chicken (85%); Mouse, Rat, Bovine (84%).
Blocking Peptide
LS-E15183 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82447. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa65-114
Specificity
Human NKD1
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NKD1 antibody LS-C82447 was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/19/2018

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