Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
Nitrotyrosine antibody was raised against purified, nitrated Keyhole Limpet Hemocyanin (KLH).
Recognizes nitrated proteins from all species.
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunohistochemistry: 5 ug/ml detects nitrated proteins in formalin-fixed paraffin embedded lateral ischemic rat brain tissue. Has been reported to detect nitrotyrosine-containing proteins in acutely injured human lung tissue. Immunocytochemistry: 5-10 ug/ml detects nitrated proteins from human A431 cells pretreated 5 minutes with 5-10 mM peroxynitrite.
0.1 M Tris-glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.