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Anti-NIT1 Antibody (aa71-120) LS-C107914

Catalog Size Price
LS-C107914-50 50 µg Unavailable

Related Products

24 NIT1 Antibodies

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Anti-NIT1 Antibody (clone 4D12) LS-C115078
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IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
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Immunohistochemistry Image
Anti-NIT1 Antibody (clone 3A11) LS-C115125
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Anti-NIT1 Antibody (aa298-327) LS-C164881
Rabbit Polyclonal to Human NIT1
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Anti-NIT1 Antibody (clone 1C3) LS-C197785
Mouse Monoclonal [clone 1C3] (IgG1,k) to Human NIT1
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Anti-NIT1 Antibody LS-C401890
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100% Guaranteed
Rabbit Polyclonal (IgG) to Human NIT1
Human, Monkey, Bat, Dog, Guinea pig, Horse, Pig
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NIT1
Rabbit
Human, Monkey, Bat, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Hamster, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NIT1 antibody was raised against synthetic peptide located between aa71-120 of human NIT1 (Q86X76, NP_005591). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog, Bat, Horse, Opossum, Guinea pig (100%); Mouse, Rat, Hamster, Bovine, Rabbit (92%); Pig (85%).
LS-E20596 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C107914. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa71-120
Human NIT1
Western Blot: Suggested dilution at 1.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NIT1

Q86X76 NM_005600 NP_005591.1

NIT1 Antibody, Nitrilase 1 Antibody, Nitrilase homolog 1 Antibody

Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.

Western blot

Western blot
NIT1 antibody LS-C107914 Western blot of Fetal Small Intestine lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 12/16/2017

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