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Anti-NIP3 / BNIP3 Antibody (aa152-187) LS-C98865

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular NIP3 / BNIP3 Antibodies

Anti-NIP3 / BNIP3 Antibody (aa152-187) LS-C98865
Rabbit Polyclonal to Human NIP3 / BNIP3
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NIP3 / BNIP3 Antibody (aa112-124) IHC-plus™ LS-B3305
Mouse Monoclonal (IgG2b) to Human NIP3 / BNIP3
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human NIP3 / BNIP3
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human NIP3 / BNIP3
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:1000)

  • IHC - Paraffin

Specificity and Use

NIP3 / BNIP3 antibody was raised against kLH-conjugated synthetic peptide between amino acids 152-187 from human BNIP3.
LS-E2279 - Lyophilized - 100 µg - $145.00
aa152-187

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
Chronic autophagy is a cellular adaptation to tumor acidic pH microenvironments. Wojtkowiak JW, Rothberg JM, Kumar V, Schramm KJ, Haller E, Proemsey JB, Lloyd MC, Sloane BF, Gillies RJ. Cancer research. 2012 72:3938-47. [PubMed:22719070]

About NIP3 / BNIP3

Q12983 NM_004052 NP_004043.3

BNIP3 Antibody, NIP3 Antibody

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody. Data courtesy of Ruben Zamora, University of Pittsburgh.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated BNIP3 (BH3 Domain Specific) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.

Western blot

Western blot
The anti-NIP3 BH3 domain antibody is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).

Requested From: United States
Date Requested: 12/5/2016

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