LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Research Areas
  • Cancer Research Resources and Products:
  • Breast Cancer
  • Colorectal Cancer
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-NFI / NFIC Antibody (clone 1C12-2A2) LS-C133344

Catalog Size Price
LS-C133344-100 100 µg (1 mg/ml) Unavailable

Related Products

39 NFI / NFIC Antibodies

Most Popular NFI / NFIC Antibodies

Anti-NFI / NFIC Antibody (aa323-372) IHC-plus™ LS-B4029
Rabbit Polyclonal (IgG) to Human NFI / NFIC
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Pig
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-NFIC antibody IHC of normal human adrenal (adipocytes). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4029 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NFI / NFIC Antibody (clone 1C12-2A2) LS-C133344
Mouse Monoclonal [clone 1C12-2A2] (IgG1,k) to Human NFI / NFIC
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10 ug/ml]
Anti-NFI / NFIC Antibody (aa180-208) LS-C164847
Rabbit Polyclonal to Human NFI / NFIC
Human
Immunofluorescence, Western blot
Unconjugated
Fluorescent confocal image of HeLa cell stained with NFIC Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.
Anti-NFI / NFIC Antibody LS-C393509
Mouse Monoclonal to Human NFI / NFIC
Human
ICC, Western blot
Unconjugated
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse monoclonal antibody (dilution 1:200).
Anti-NFI / NFIC Antibody LS-C413018
Mouse Monoclonal to Human NFI / NFIC
Human
ICC, Western blot
Unconjugated
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse mAb (dilution 1:200).

100% Guaranteed
Mouse Monoclonal [clone 1C12-2A2] (IgG1,k) to Human NFI / NFIC
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human NFI / NFIC
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1C12-2A2]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

  • IHC - Paraffin

Specificity and Use

NFI / NFIC antibody was raised against nFIC (AAH12120, 1 a.a. ~ 429 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human NFIC
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About NFI / NFIC

P08651 NM_005597 NP_005588.2

NFIC Antibody, CTF Antibody, CTF5 Antibody, NF1-C Antibody, NF-I/C Antibody, NFI Antibody, NFI-C Antibody, TGGCA-binding protein Antibody, NF-I Antibody, Nuclear factor 1 C-type Antibody, Nuclear factor 1/C Antibody, Nuclear factor I/C Antibody

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Immunofluorescence

Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.
Western blot of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.

Western blot

NFIC monoclonal antibody (M01), clone 1C12-2A2 Western blot of NFIC expression in A-431.
NFIC monoclonal antibody (M01), clone 1C12-2A2 Western blot of NFIC expression in A-431.

RNA interference

Western blot of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody, clone 1C12-2A2. GAPDH ( 36.1 kD ) used as specific.
Western blot of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody, clone 1C12-2A2. GAPDH ( 36.1 kD ) used as specific.

Requested From: 
Date Requested: 5/24/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number