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Anti-NF-L / NEFL Antibody (clone DA2) LS-C204554

Ordering

Wt. Vol. Conc. Price
- 500 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NF-L / NEFL Antibodies

Anti-NF-L / NEFL Antibody LS-C16795
Mouse Monoclonal (IgG1,k) to Human NF-L / NEFL
Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-NF-L / NEFL Antibody IHC-plus™ LS-B257
Rabbit Polyclonal to Pig NF-L / NEFL
Pig, Human, Mouse, Rat, Bovine, Avian, Mammal
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NF-L / NEFL Antibody (clone 3G2) LS-C175341
Mouse Monoclonal [clone 3G2] (IgG1) to Human NF-L / NEFL
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone DA2] (IgG1,k) to Pig NF-L / NEFL
Pig, Human, Mouse, Rat, Bovine
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Pig NF-L / NEFL
Mouse
Pig, Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [DA2]
Unconjugated
Tissue culture supernatant
Unmodified

Applications

  • ICC (1:500)
  • Immunofluorescence (1:500)
  • Western blot (1:10000 - 1:20000)

Specificity and Use

NF-L / NEFL antibody was raised against preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H.
Reacts with NF-L from human, cow, pig, mouse, rat and all other mammals, and also recognizes avian NF-L. It is strong and clean on western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.
For immunofluorescence, use NF-L / NEFL antibody diluted 1:500. For western blots try the antibody diluted 1:10000-1:20000.

Packaging

Tissue culture supernatant
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About NF-L / NEFL

P07196 NM_006158 NP_006149.2

NEFL Antibody, 68 kDa neurofilament protein Antibody, CMT1F Antibody, CMT2E Antibody, NF68 Antibody, NF-L Antibody, NFL Antibody, Neurofilament subunit NF-L Antibody

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein.

Immunocytochemistry

Immunocytochemistry
Cells grown from adult rat brain using method described by Evans et al. (2). Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCA-a-Int (red). These cells were mitotic but had several characteristics of neurons.

Western blot

Western blot
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NF-L / NEFL antibody (lane 4).

Requested From: United States
Date Requested: 12/5/2016

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