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Anti-NF-L / NEFL Antibody (clone 7D1) LS-C204557

Note: This antibody replaces LS-C143053

Ordering

Wt. Vol. Conc. Price
- 100 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular NF-L / NEFL Antibodies

Anti-NF-L / NEFL Antibody LS-C16795
Mouse Monoclonal (IgG1,k) to Human NF-L / NEFL
Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-NF-L / NEFL Antibody IHC-plus™ LS-B257
Rabbit Polyclonal to Pig NF-L / NEFL
Pig, Human, Mouse, Rat, Bovine, Avian, Mammal
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NF-L / NEFL Antibody (clone 3G2) LS-C175341
Mouse Monoclonal [clone 3G2] (IgG1) to Human NF-L / NEFL
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 7D1] (IgG2b) to Human NF-L / NEFL
Human, Mouse, Rat, Bovine, Pig
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human NF-L / NEFL
Mouse
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [7D1]
Unconjugated
Ascites
Unmodified

Applications

  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:5000 - 1:10000)

Specificity and Use

NF-L / NEFL antibody was raised against preparation of pig neurofilament NF-H and NF-L.
Reacts with NF-L from human, cow, pig, mouse, rat and all other mammals. It is strong and clean on western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.
For immunofluorescence, use NF-L / NEFL antibody diluted 1:100-1:500. For western blots try the antibody diluted 1:5000-1:10000.

Packaging

Ascites
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About NF-L / NEFL

P07196 NM_006158 NP_006149.2

NEFL Antibody, 68 kDa neurofilament protein Antibody, CMT1F Antibody, CMT2E Antibody, NF68 Antibody, NF-L Antibody, NFL Antibody, Neurofilament subunit NF-L Antibody

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein.

Immunocytochemistry

Immunocytochemistry
Embryonic rat cortical cells grown in tissue culture and stained with NF-L / NEFL antibody (green) and the phosphorylated axonal form of neurofilament NF-H, using NF-H antibody (red). The perikaryal and dendritic neurofilaments in the Large cell in middle are stained with NF-L / NEFL antibody but not with the phosphorylated NF-H antibody. In contrast both antibodies stain axonal neurofilaments which therefore appear orange.

Western blot

Western blot
Strip blots of rat spinal cord homogenate probed with two subclones of NF-L / NEFL antibody. Both subclones, one of which became the definitive antibody, stain strongly and specifically a band at 68 kDa with essentially no background.

Requested From: United States
Date Requested: 12/9/2016

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