Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
Chick retina cells.
Recognizes chicken Neuron Cell Surface Antigen A2B5. Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Species cross-reactivity: human.
Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Indirect Immunofluorescence: 1:100-1:500. For the detection of neurons in tissue culture. Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments. Immunohistochemistry: Frozen, fixed tissues. Immunocytochemistry: Confocal fluorescent analysis of PC12 cells. Positive control: Type II astrocytes, human neural progenitors, PC12 cells.