Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [A60]
Biotin
Protein A purified
Unmodified
Applications
IHC
IHC - Paraffin
ICC (1:10 - 1:500)
Western blot
Specificity and Use
NeuN antibody was raised against purified cell nuclei from mouse brain.
Reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive include Purkinje, mitral and photoreceptor cells. Species cross-reactivity: Rat. It is expected that it will also react with human, ferret, chick and salamander.
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry: 1:100-1:2000; Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde-based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD. Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% triton X-100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Packaging
PBS, pH 7.1, 0.09% sodium azide, 15 mg/ml BSA.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
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