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Anti-NeuN Antibody (clone A60, Biotin) LS-C73395

Catalog Size Price
LS-C73395-500 500 µg (1 mg/ml) Unavailable

Related Products

8 NeuN Antibodies

Most Popular NeuN Antibodies

Anti-NeuN Antibody (clone A60) LS-C73393
Mouse Monoclonal [clone A60] (IgG1) to Mouse NeuN
Mouse, Human, Rat, Ferret, Chicken
ICC, Western blot
Anti-NeuN Antibody (clone A60, Biotin) LS-C73395
Mouse Monoclonal [clone A60] (IgG1) to Mouse NeuN
Mouse, Rat
IHC, IHC - Paraffin, ICC, Western blot
Biotin Conjugated
Anti-NeuN Antibody LS-C149132
Chicken Polyclonal
Human, Mouse
Anti-NeuN Antibody (clone A60) LS-C312122
Mouse Monoclonal [clone A60] (IgG1) to Human NeuN
Human, Mouse, Rat, Ferret, Pig, Chicken, Avian, Salamander
IHC, IHC - Paraffin, IHC - Frozen
IHC of NeuN on FFPE Brain tissue.
Anti-NeuN Antibody (aa80-130) LS-C483047
Rabbit Polyclonal to Human NeuN
Human, Mouse, Rat
IHC, Western blot
Rabbit antibody to NeuN (80-130). IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

100% Guaranteed
Mouse Monoclonal [clone A60] (IgG1) to Mouse NeuN
Mouse, Rat
IHC, IHC - Paraffin, ICC, Western blot
Biotin Conjugated


Mouse NeuN
Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [A60]
Protein A purified


  • IHC
  • IHC - Paraffin
  • ICC (1:10 - 1:500)
  • Western blot

Specificity and Use

NeuN antibody was raised against purified cell nuclei from mouse brain.
Reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive include Purkinje, mitral and photoreceptor cells. Species cross-reactivity: Rat. It is expected that it will also react with human, ferret, chick and salamander.
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry: 1:100-1:2000; Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde-based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD. Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% triton X-100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.


PBS, pH 7.1, 0.09% sodium azide, 15 mg/ml BSA.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About NeuN

Requested From: 
Date Requested: 4/25/2018

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