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Anti-NeuN Antibody (clone A60, Biotin) LS-C73395


Wt. Vol. Conc. Price
500 µg - 1 mg/ml $590
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone A60] (IgG1) to Mouse NeuN
Mouse, Rat
IHC - Paraffin, ICC, Western blot
Biotin Conjugated


Mouse NeuN
Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [A60]
Protein A purified


  • IHC - Paraffin
  • ICC (1:10 - 1:500)
  • Western blot

Specificity and Use

NeuN antibody was raised against purified cell nuclei from mouse brain.
Reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive include Purkinje, mitral and photoreceptor cells. Species cross-reactivity: Rat. It is expected that it will also react with human, ferret, chick and salamander.
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry: 1:100-1:2000; Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde-based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD. Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% triton X-100.


PBS, pH 7.1, 15 mg/ml BSA, 0.1% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About NeuN

Requested From: United States
Date Requested: 10/26/2016

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