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Anti-NEK7 Antibody (aa74-123) LS-C81339

Catalog Size Price
LS-C81339-50 50 µg Unavailable

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28 NEK7 Antibodies

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Anti-NEK7 antibody LS-A7163 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 Antibody (aa29-39) IHC-plus™ LS-B8348
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Anti-NEK7 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8348 dilution 3.75 ug/ml.
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Detection of Human NEK7 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NEK7 used at a dilution of 1:1000 (1 Detection: DAB.
Anti-NEK7 Antibody (aa1-30) IHC-plus™ LS-B10926
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Anti-NEK7 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10926 dilution 1:50.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human NEK7
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Chicken, Xenopus, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NEK7
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

NEK7 antibody was raised against synthetic peptide located between aa74-123 of human NEK7 (Q8TDX7, NP_598001). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Salmon, Stickleback, Zebrafish (100%); Rabbit, Sea squirt (92%); Gorilla, Orangutan (85%); Toxoplasma (80%).
LS-E17725 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81339. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa74-123
Human NEK7
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About NEK7

Q8TDX7 NM_133494 NP_598001.1

NEK7 Antibody, NimA-related protein kinase 7 Antibody, NIMA-related kinase 7 Antibody

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/20/2018

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