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Anti-NEK7 Antibody (aa238-302) LS-C185958

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.85 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NEK7 Antibodies

Anti-NEK7 Antibody (N-Terminus) IHC-plus™ LS-A7163
Rabbit Polyclonal to Human NEK7
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NEK7
Human, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human NEK7
Rabbit
Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

NEK7 antibody was raised against synthetic peptide corresponding to a region within amino acids 238 and 302 of NEK7 (SwissProt Q8TDX7). Percent identity by BLAST analysis: Human, Rat (100%).
aa238-302
Human NEK7
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About NEK7

Q8TDX7 NM_133494 NP_598001.1

NEK7 Antibody, NimA-related protein kinase 7 Antibody, NIMA-related kinase 7 Antibody

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Tw2.6 xenograft using NEK7 antibody at 1:100 dilution.

Immunocytochemistry

Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using NEK7 antibody at 1:200 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: HeLa S3. 12% SDS PAGE. NEK7 antibody diluted at 1:500
 

Requested From: United States
Date Requested: 12/11/2016

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