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Anti-NEI3 / NEIL3 Antibody (aa164-177) IHC-plus™ LS-B259

Note: This antibody replaces LS-C2718, LS-C25737

Ordering

Wt. Vol. Conc. Price
- 100 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NEI3 / NEIL3 Antibodies

Anti-NEI3 / NEIL3 Antibody (aa164-177) IHC-plus™ LS-B259
Rabbit Polyclonal to Human NEI3 / NEIL3
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NEI3 / NEIL3 Antibody (aa164-177) IHC-plus™ LS-B4815
Rabbit Polyclonal (IgG) to Human NEI3 / NEIL3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NEI3 / NEIL3 Antibody (aa491-540) IHC-plus™ LS-B13433
Rabbit Polyclonal (IgG) to Human NEI3 / NEIL3
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NEI3 / NEIL3
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human NEI3 / NEIL3
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500)

Specificity and Use

NEI3 / NEIL3 antibody was raised against residues 164-177 (LRAESEVKKQKGRMLG) of the human NEI3 protein. 85% sequence identity to mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Marmoset (100%); Gibbon, Monkey, Elephant (94%); Panda, Dog, Bovine, Horse (88%); Chicken (81%).
aa164-177
Human NEI3.
Immunohistochemistry: LS-B259 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B259 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.02 % sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NEI3 / NEIL3

Q8TAT5 NM_018248 NP_060718.2

NEIL3 Antibody, DNA glycosylase/AP lyase Neil3 Antibody, FGP2 Antibody, DNA glycosylase FPG2 Antibody, DNA glycosylase hFPG2 Antibody, FPG2 Antibody, HFPG2 Antibody, Endonuclease 8-like 3 Antibody, Endonuclease VIII-like 3 Antibody, HNEI3 Antibody, Nei-like protein 3 Antibody, NEI3 Antibody

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity.

Immunohistochemistry

Immunohistochemistry
Anti-NEIL3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B259 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/8/2016

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