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Anti-NEFM / NF-M Antibody (C-Terminus) IHC-plus™ LS-B258

Note: This antibody replaces LS-C2727


Wt. Vol. Conc. Price
- 100 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NEFM / NF-M Antibodies

Anti-NEFM / NF-M Antibody (clone RNF403) IHC-plus™ LS-B7183
Mouse Monoclonal [clone RNF403] (IgG1) to Human NEFM / NF-M
Human, Monkey, Hamster, Xenopus
IHC - Paraffin, IHC - Frozen, Western blot
Immunohistochemistry Image
Anti-NEFM / NF-M Antibody (clone RNF406) IHC-plus™ LS-B7184
Mouse Monoclonal [clone RNF406] (IgG1) to Human NEFM / NF-M
Human, Monkey, Guinea pig, Hamster, Rabbit, Xenopus
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Immunohistochemistry Image
Anti-NEFM / NF-M Antibody (aa250-263) IHC-plus™ LS-B11174
Goat Polyclonal to Human NEFM / NF-M
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Rat NEFM / NF-M
Rat, Human, Mouse, Bovine, Cat, Pig, Avian
IHC - Paraffin, IHC - Frozen, Western blot


Rat, Human, Mouse, Bovine, Cat, Pig, Avian (tested or 100% immunogen sequence identity)


  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen
  • Western blot (1:5000)

Specificity and Use

NEFM / NF-M antibody was raised against recombinant C-terminal segment of rat NF-M
Specifically recognizes the evolutionarily conserved extreme C-terminal region of Neurofilament Medium (~145-170kD).
Immunohistochemistry: LS-B258 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B258 was determined to be 5 ug/ml.


Long term: -70°C; Short term: +4°C
For research use only.

About NEFM / NF-M

P07197 NM_005382 NP_005373.2

NEFM Antibody, 160 kDa neurofilament protein Antibody, NEF3 Antibody, NF-M Antibody, NFM Antibody, Neurofilament 3 Antibody

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.


Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B258 concentration 5 ug/ml.

Requested From: United States
Date Requested: 10/25/2016

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