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Antibodies > NEFH / NF-H > LS-C204553
 
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Catalog Number Size Price
LS-C204553-100 100 µl $376 
NEFH / NF-H Antibody - Shows a section of rat spinal cord stained with NEFH / NF-H antibody (green) and monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1) clone MCA-BH7 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
NEFH / NF-H Antibody - Lane labeled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated. In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. The Rab lane shows a blot probed with rabbit anti NF-H antibody NEFH / NF-H antibody. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H, CPCA-NF-H.
NEFH / NF-H Antibody - Shows a section of rat spinal cord stained with NEFH / NF-H antibody (green) and monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1) clone MCA-BH7 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
NEFH / NF-H Antibody - Lane labeled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated. In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. The Rab lane shows a blot probed with rabbit anti NF-H antibody NEFH / NF-H antibody. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H, CPCA-NF-H.
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Polyclonal Rabbit anti‑Bovine NEFH / NF‑H Antibody (IHC, IF, WB) LS‑C204553

Polyclonal Rabbit anti‑Bovine NEFH / NF‑H Antibody (IHC, IF, WB) LS‑C204553

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Antibody:
NEFH / NF-H Rabbit anti-Bovine Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Bovine, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204553-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NEFH / NF-H Rabbit anti-Bovine Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Bovine, Human
Format:
Unconjugated, Unmodified

Specifications

Description
NF-H antibody LS-C204553 is an unconjugated rabbit polyclonal antibody to NF-H (NEFH) from bovine. It is reactive with human and bovine. Validated for IF, IHC and WB.
Target
Bovine NEFH / NF-H
Synonyms
NEFH | 200 kDa neurofilament protein | KIAA0845 | NFH | NF-H
Host
Rabbit
Reactivity
Bovine, Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
Purified bovine NF-H
Specificity
Bovine NEFH / NF-H
Applications
  • IHC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
We suggest a dilution of 1:1000 for immunofluorescence microscopy and 1:10000 for western blots. The antibody has also been used successfully in the detection role in ELISA.
Presentation
Antiserum
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEFH / NF-H
P12036 NM_021076 NP_066554.2

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NEFH / NF-H Antibody - Anti-NEFH antibody IHC of human peripheral nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ NEFH / NF‑H Antibody (clone NF‑05) LS‑B4502
Species: Human, Rat, Porcine
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
NEFH / NF-H Antibody - Western blot of rat cortex lysate showing specific immunolabeling of the ~200k NF-H protein.
NEFH / NF‑H Antibody LS‑C61817
Species: Bovine, Chicken, Mammal
Applications: Immunofluorescence, Western blot
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NEFH / NF‑H Antibody LS‑C37558
Species: Rat, Human
Applications: IHC, Western blot
NEFH / NF-H Antibody - Human Testis, Nerve Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ NEFH / NF‑H Antibody LS‑A12537
Species: Human
Applications: IHC - Paraffin
NEFH / NF-H Antibody - Immunostaining of cultured neurons and glia with anti-chicken neurofilament H antibody (red) and anti-neurofilament L antibody (green).
NEFH / NF‑H Antibody LS‑C61818
Species: Bovine, Human, Mouse, Rat, Chicken
Applications: ICC, Immunofluorescence, Western blot
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NEFH / NF‑H Antibody LS‑C755081
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
See all NEFH / NF-H Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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