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Anti-NEFH / NF-H Antibody LS-C204555

Ordering

Wt. Vol. Conc. Price
- 100 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NEFH / NF-H Antibodies

Anti-NEFH / NF-H Antibody (C-Terminus) IHC-plus™ LS-B256
Rabbit Polyclonal to Rat NEFH / NF-H
Rat, Human, Mouse, Bovine, Cat, Pig
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NEFH / NF-H Antibody (clone NF-05) IHC-plus™ LS-B4502
Mouse Monoclonal [clone NF-05] (IgG1) to Human NEFH / NF-H
Human, Rat, Pig
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NEFH / NF-H Antibody LS-C414328
Rabbit Polyclonal to Human NEFH / NF-H
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NEFH / NF-H
Human
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human NEFH / NF-H
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)

Specificity and Use

NEFH / NF-H antibody was raised against purifed NF-H construct containing most of the tandem KSP repeats.
Human NEFH / NF-H
We suggest a dilution of 1:1000 for immunofluorescence microscopy and 1:10000 for western blots. The antibody has also been used successfully in the detection role in ELISA.

Packaging

Antiserum
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About NEFH / NF-H

P12036 NM_021076 NP_066554.2

NEFH Antibody, 200 kDa neurofilament protein Antibody, KIAA0845 Antibody, NFH Antibody, NF-H Antibody

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Immunocytochemistry

Immunocytochemistry
Shows mixed neuron/glial rat brain cultures stained with NEFH / NF-H antibody (green) and chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP) (red). The neurofilament NF-H antibody binds to phosphorylated axonal forms of NF-H and non-phosphorylated dendritic and perikaryal forms.

Western blot

Western blot
Blot of rat spinal cord extract blotted with NEFH / NF-H antibody. A major band running at 200kDa is NF-H, the major neurofilament subunit protein. A minor band at about 160kDa is the non-phosphorylated dendritic and perilaryal form of this protein.

Requested From: United States
Date Requested: 12/8/2016

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