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Catalog Number	Size	Price
LS-C204550-100	100 µl	$376

NEFH / NF-H Antibody - Mixed neuron/glial cultures stained with NEFH / NF-H antibody (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L RPCA-NF-L (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NEFH / NF-H antibody binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.

NEFH / NF-H Antibody - Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, MCA-NAP4 (lane 1), NEFH / NF-H antibody (Lane 2) and MCA-9B12 (lane 3). All three antibodies bind to a prominent band with an apparent SDS-PAGE molecular weight of 200kDa.

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Monoclonal Mouse anti‑Bovine NEFH / NF‑H Antibody (clone AH1, IF, WB) LS‑C204550

Name: Anti-NEFH / NF-H Antibody | Mouse anti-Bovine Monoclonal ICC,IF,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C204550-100
Price: 376.0000 USD
Availability: InStock

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Antibody:

NEFH / NF-H Mouse anti-Bovine Monoclonal (AH1) Antibody

Application:

ICC, IF, WB

Reactivity:

Bovine, Chicken, Mammal

Format:

Unconjugated, Unmodified

$376

LS-C204550-100

Toll Free North America

206-374-1102

For Research Use Only

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Overview

Antibody:

NEFH / NF-H Mouse anti-Bovine Monoclonal (AH1) Antibody

Application:

ICC, IF, WB

Reactivity:

Bovine, Chicken, Mammal

Format:

Unconjugated, Unmodified

Specifications

Description

NF-H antibody LS-C204550 is an unconjugated mouse monoclonal antibody to NF-H (NEFH) from bovine. It is reactive with chicken and mammal. Validated for ICC, IF and WB.

Target

Bovine NEFH / NF-H

Synonyms

NEFH | 200 kDa neurofilament protein | KIAA0845 | NFH | NF-H

See All NEFH / NF-H Antibodies

Host

Mouse

Reactivity

Bovine, Chicken, Mammal (tested or 100% immunogen sequence identity)

Clonality

IgG1,k Monoclonal

Clone

AH1

Conjugations

Unconjugated

Purification

Ascites

Modifications

Unmodified

Immunogen

Native NF-H purified from bovine spinal cord.

Specificity

Clone AH1 recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

Applications

ICC (1:1000)
Immunofluorescence (1:1000)
Western blot (1:10000)

Usage

The ascites solution has a high titre and can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.

Presentation

Ascites fluid.

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About NEFH / NF-H

Uniprot: P12036 NCBI: NM_021076 NP_066554.2