Target
Human NEBL LASP2
Synonyms
NEBL | Actin-binding Z-disk protein | LASP2 | LIM-nebulette | LNEBL | Nebulette
Reactivity
Human, Monkey, Mouse, Chicken
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-ELQRLKRTQE, from the internal region of the protein sequence according to NP_998734.1NP_001166955.1.
Specificity
Human NEBL. This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave an approx 30 kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1 ug/ml antibody staining. This protein has a calculated MW of 31.2 kDa according to NP_998734.1. The 30 kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee