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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NDUFB4 / B15 Antibody LS‑C155314 Best Seller
B15 antibody LS-C155314 is an unconjugated rabbit polyclonal antibody to B15 (NDUFB4) from human and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C155314-100
100 µl (1 mg/ml)
Unavailable

Popular NDUFB4 / B15 Products

 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot
Immunohistochemical of paraffin-embedded H. skin section using NDUFB4 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Western blot of NDUFB4 Antibody with human Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon
Applications: Western blot

Product Description

B15 antibody LS-C155314 is an unconjugated rabbit polyclonal antibody to B15 (NDUFB4) from human and rat. Validated for IHC and WB.
About NDUFB4 / B15
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. O95168 NM_004547 NP_004538.2

NDUFB4 Antibody, B15 Antibody, Complex I B15 subunit Antibody, CI-B15 Antibody, Complex I-B15 Antibody


Specifications

Target
Human NDUFB4 / B15
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NDUFB4 / B15 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 129 of NDUFB4 (SwissProt O95168). Percent identity by BLAST analysis:
Specificity
Human, rat NDUFB4
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.
IHC of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.

Immunohistochemistry

IHC of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.
IHC of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE NDUFB4 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE NDUFB4 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.
IHC of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.

Immunohistochemistry

IHC of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.
IHC of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE NDUFB4 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE NDUFB4 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.
IHC of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.

Immunohistochemistry

IHC of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.
IHC of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE NDUFB4 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE NDUFB4 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.
IHC of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.

Immunohistochemistry

IHC of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.
IHC of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE NDUFB4 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE NDUFB4 antibody diluted at 1:1000


Requested From: 
Date Requested: 6/20/2018

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