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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NCOA1 / SRC‑1 Antibody (aa477‑947) LS‑C10342
SRC-1 antibody LS-C10342 is an unconjugated mouse monoclonal antibody to SRC-1 (NCOA1) from human, mouse, rat and other species. Validated for IP and WB.
Catalog
Size
Price
LS-C10342-100
100 µg
$605
Description
SRC-1 antibody LS-C10342 is an unconjugated mouse monoclonal antibody to SRC-1 (NCOA1) from human, mouse, rat and other species. Validated for IP and WB.
Target
Human NCOA1 / SRC-1
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
NCOA1 / SRC-1 antibody was raised against gST fusion-protein containing amino acids 477-947 of human Src-1.
Epitope
aa477-947
Specificity
Recognizes SRC-1 isoforms at ~160kD. May also weakly recognize unidentified proteins at 120, 45 and 40kD. Species cross-reactivity: Human, monkey, mouse and rat.
Usage
Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects Src-1 in HeLa and Raji Nuclear Extract cells. A previous lot of detected SRC-1 in a RIPA lysate from Cos cells. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SRC-1 (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 ug.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About NCOA1 / SRC-1
Q15788 NM_003743 NP_003734.3

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Requested From: United States
Date Requested: 11/17/2018
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