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Anti-NCOA1 / SRC-1 Antibody (aa477-947) LS-C10342

Ordering

Wt. Vol. Conc. Price
100 µg - - $485
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Mouse Monoclonal (IgG1,k) to Human NCOA1 / SRC-1
Human, Monkey, Mouse, Rat
Western blot, Immunoprecipitation
Unconjugated

Details

Human NCOA1 / SRC-1
Mouse
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Protein G purified

Applications

Western blot (0.5 - 2 µg/ml), Immunoprecipitation

Specificity and Use

NCOA1 / SRC-1 antibody was raised against gST fusion-protein containing amino acids 477-947 of human Src-1.
aa477-947
Recognizes SRC-1 isoforms at ~160kD. May also weakly recognize unidentified proteins at 120, 45 and 40kD. Species cross-reactivity: Human, monkey, mouse and rat.
Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects Src-1 in HeLa and Raji Nuclear Extract cells. A previous lot of detected SRC-1 in a RIPA lysate from Cos cells. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SRC-1 (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 ug.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NCOA1 / SRC-1

Q15788 NM_003743 NP_003734.3

NCOA1 Antibody, BHLHe42 Antibody, BHLHe74 Antibody, F-SRC-1 Antibody, Hin-2 protein Antibody, Nuclear receptor coactivator 1 Antibody, PAX3/NCOA1 fusion protein Antibody, KAT13A Antibody, Protein Hin-2 Antibody, Steroid receptor coactivator 1 Antibody, Steroid receptor coactivator-1 Antibody, NCoA-1 Antibody, RIP160 Antibody, SRC-1 Antibody, SRC1 Antibody

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors.

Requested From: United States
Date Requested: 9/26/2016

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