Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
NCOA1 / SRC-1 antibody was raised against gST fusion-protein containing amino acids 477-947 of human Src-1.
Recognizes SRC-1 isoforms at ~160kD. May also weakly recognize unidentified proteins at 120, 45 and 40kD. Species cross-reactivity: Human, monkey, mouse and rat.
Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects Src-1 in HeLa and Raji Nuclear Extract cells. A previous lot of detected SRC-1 in a RIPA lysate from Cos cells. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SRC-1 (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 ug.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors.