SRC-1 antibody LS-C10342 is an unconjugated mouse monoclonal antibody to SRC-1 (NCOA1) from human, mouse, rat and other species. Validated for IP and WB.
Human NCOA1 / SRC-1
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
NCOA1 / SRC-1 antibody was raised against gST fusion-protein containing amino acids 477-947 of human Src-1.
Recognizes SRC-1 isoforms at ~160kD. May also weakly recognize unidentified proteins at 120, 45 and 40kD. Species cross-reactivity: Human, monkey, mouse and rat.
Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects Src-1 in HeLa and Raji Nuclear Extract cells. A previous lot of detected SRC-1 in a RIPA lysate from Cos cells. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SRC-1 (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 ug.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.