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Anti-NCKAP1 / NAP125 Antibody (N-Terminus) LS-C155424

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.82 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NCKAP1 / NAP125 Antibodies

Anti-NCKAP1 / NAP125 Antibody (aa703-715) LS-C155062
Goat Polyclonal to Human NCKAP1 / NAP125
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
ELISA
Unconjugated
Anti-NCKAP1 / NAP125 Antibody (aa631-659, AP) LS-C264093
Rabbit Polyclonal (IgG) to Human NCKAP1 / NAP125
Human
Western blot, ELISA
AP Conjugated
Anti-NCKAP1 / NAP125 Antibody (aa631-659, Biotin) LS-C264096
Rabbit Polyclonal (IgG) to Human NCKAP1 / NAP125
Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NCKAP1 / NAP125
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human NCKAP1 / NAP125
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

NCKAP1 / NAP125 antibody was raised against recombinant fragment corresponding to a region within amino acids 2 and 338 of NCKAP1 (SwissProt Q9Y2A7). Percent identity by BLAST analysis: Human (100%); Mouse (98%); Rat (97%); Xenopus (94%); Zebrafish (92%).
N-Terminus
Human NCKAP1
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Packaging

PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
For research use only.

About NCKAP1 / NAP125

Q9Y2A7 NM_013436 NP_038464.1

NCKAP1 Antibody, HEM2 Antibody, KIAA0587 Antibody, NAP1 Antibody, NAP125 Antibody, p125Nap1 Antibody, NAP 1 Antibody, NCK-associated protein 1 Antibody

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Lung Papillary Adenocarcinoma, using NCKAP1 antibody at 1:250 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE NCKAP1 antibody diluted at 1:1000

Requested From: United States
Date Requested: 12/3/2016

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