Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
ICC (1:250 - 1:2000)
Western blot (1:250 - 1:1000)
GST fusion protein corresponding to full-length human p47-phox.
Recognizes human p47-phox, Mr 47kD. Species cross-reactivity: Reported cross-reactivity with bovine.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:250-1:1000 detected p47-phox in RIPA lysates from HL-60 cells. Immunoprecipitation: 10 ul immunoprecipitates p47-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:250-1:2000 dilution showed positive cytosolic immunostaining for p47-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
5 mM EDTA, 0.02% sodium azide, 40% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
NCF1 encodes neutrophil cytosolic factor 1, the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.