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Anti-NCF1 / p47phox / p47 phox Antibody LS-C9427

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Rabbit Polyclonal (IgG) to Human NCF1 / p47phox / p47 phox
Human, Bovine
ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human NCF1 / p47phox / p47 phox
Rabbit
Human, Bovine (tested or 100% immunogen sequence identity)
IgG Polyclonal
Antiserum

Applications

ICC (1:250 - 1:2000), Western blot (1:250 - 1:1000), Immunoprecipitation

Specificity and Use

NCF1 / p47phox / p47 phox antibody was raised against gST fusion protein corresponding to full-length human p47-phox.
Recognizes human p47-phox, Mr 47kD. Species cross-reactivity: Reported cross-reactivity with bovine.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:250-1:1000 detected p47-phox in RIPA lysates from HL-60 cells. Immunoprecipitation: 10 ul immunoprecipitates p47-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:250-1:2000 dilution showed positive cytosolic immunostaining for p47-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

Packaging

5 mM EDTA, 0.02% sodium azide, 40% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NCF1 / p47phox / p47 phox

P14598 NM_000265 NP_000256.4

NCF1 Antibody, NCF1A Antibody, Neutrophil cytosolic factor 1 Antibody, NCF-47K Antibody, Nox organizer 2 Antibody, NOXO2 Antibody, p47-phox Antibody, p47phox Antibody, SH3PXD1A Antibody, NADPH oxidase organizer 2 Antibody, NCF-1 Antibody, Neutrophil cytosol factor 1 Antibody, Nox-organizing protein 2 Antibody

NCF1 encodes neutrophil cytosolic factor 1, the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Requested From: United States
Date Requested: 9/26/2016

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