P47 phox antibody LS-C9427 is an unconjugated rabbit polyclonal antibody to p47 phox (NCF1 / p47phox) from human and bovine. Validated for ICC, IP and WB.
Human NCF1 / p47phox / p47 phox
(tested or 100% immunogen sequence identity)
- ICC (1:250 - 1:2000)
- Western blot (1:250 - 1:1000)
GST fusion protein corresponding to full-length human p47-phox.
Recognizes human p47-phox, Mr 47kD. Species cross-reactivity: Reported cross-reactivity with bovine.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:250-1:1000 detected p47-phox in RIPA lysates from HL-60 cells. Immunoprecipitation: 10 ul immunoprecipitates p47-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:250-1:2000 dilution showed positive cytosolic immunostaining for p47-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
5 mM EDTA, 0.02% sodium azide, 40% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About NCF1 / p47phox / p47 phox