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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NCF1 / p47phox / p47 phox Antibody LS‑C9427
P47 phox antibody LS-C9427 is an unconjugated rabbit polyclonal antibody to p47 phox (NCF1 / p47phox) from human and bovine. Validated for ICC, IP and WB.
Antibody
Catalog
Size
Price
LS-C9427-200
200 µl
$695

Popular NCF1 / p47phox / p47 phox Products

Anti-p47phox antibody IHC of human colon, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2365 concentration 5 ug/ml.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-p47phox antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2549 concentration 10 ug/ml.
Species: Mouse, Human
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Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Sample (50 ug of whole cell lysate) A: mouse spleen 10% SDS PAGE p47phox antibody diluted at 1:500
Species: Human, Mouse
Applications: Western blot

Product Description

P47 phox antibody LS-C9427 is an unconjugated rabbit polyclonal antibody to p47 phox (NCF1 / p47phox) from human and bovine. Validated for ICC, IP and WB.

Specifications

Target
Human NCF1 / p47phox / p47 phox
Synonyms
NCF1, NCF1A, Neutrophil cytosolic factor 1, NCF-47K, Nox organizer 2, NOXO2, p47-phox, p47phox, SH3PXD1A, NADPH oxidase organizer 2, NCF-1, Neutrophil cytosol factor 1, Nox-organizing protein 2
Host
Rabbit
Reactivity
Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • ICC (1:250 - 1:2000)
  • Western blot (1:250 - 1:1000)
  • Immunoprecipitation
Immunogen
GST fusion protein corresponding to full-length human p47-phox.
Specificity
Recognizes human p47-phox, Mr 47kD. Species cross-reactivity: Reported cross-reactivity with bovine.
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:250-1:1000 detected p47-phox in RIPA lysates from HL-60 cells. Immunoprecipitation: 10 ul immunoprecipitates p47-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:250-1:2000 dilution showed positive cytosolic immunostaining for p47-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Presentation
5 mM EDTA, 0.02% sodium azide, 40% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About NCF1 / p47phox / p47 phox
NCF1 encodes neutrophil cytosolic factor 1, the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease. P14598 NM_000265 NP_000256.4


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Requested From: United States
Date Requested: 10/16/2018

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