NANP antibody LS-A3355 is an unconjugated rabbit polyclonal antibody to NANP from human and monkey. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
NANP antibody was raised against synthetic 16 amino acid peptide from N-terminus of human NANP. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Rat, Bovine, Bat, Hamster, Panda, Pig (88%); Mouse, Dog, Elephant, Turkey, Chicken, Platypus (81%).
Human NANP. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A3355 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A3355 was determined to be 15-20 ug/ml.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.