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Anti-NANOG Antibody (phospho-Ser71) LS-C163646

Catalog Size Price
LS-C163646-400 400 µl Unavailable

Related Products

108 NANOG Antibodies

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Anti-NANOG Antibody (clone hNanog.2) IHC-plus™ LS-B7871
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100% Guaranteed
Rabbit Polyclonal to Human NANOG
Human
ICC, Immunofluorescence, Dot Blot
Unconjugated

Details

Human NANOG
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • ICC
  • Immunofluorescence (1:10 - 1:50)
  • Dot Blot (1:500)

Specificity and Use

LS-E7447 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C163646. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
pSer71
This Nanog Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S71 of human Nanog.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About NANOG

Q9H9S0 AB062943 NP_079141.2

NANOG Antibody, Nanog homeobox Antibody, NANOGP8 Antibody, HNanog Antibody, Homeobox protein NANOG Antibody

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of SY5Y cells stained Phospho-Nanog-S71 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated Phospho-Nanog-S71 primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Phospho-Nanog immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.

Dot Blot

Dot Blot
Dot blot of anti-Phospho-Nanog-S71 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Requested From: 
Date Requested: 8/18/2017

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