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Anti-NACA Antibody (C-Terminus) IHC-plus™ LS-B2470

Note: This antibody replaces LS-C16713

Ordering

Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NACA Antibodies

Anti-NACA Antibody (C-Terminus) IHC-plus™ LS-B2470
Rabbit Polyclonal (IgG) to Human NACA
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal (IgG) to Human NACA
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human NACA
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 2 µg/ml)

Specificity and Use

NACA antibody was raised against synthetic peptide derived from the C-terminal region of the human alpha-NAC protein.
C-Terminus
Recognizes the human alpha-NAC protein. On Western blots, it identifies a single band at ~23kD. Species cross-reactivity: mouse and rat.
Immunohistochemistry: LS-B2470 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2470 was determined to be 5 ug/ml. Positive control: Fetal mouse brain, rat brain homogenates and human NGP96 cell lysate.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NACA

Q13765 NM_005594 NP_005585.1

NACA Antibody, Allergen Hom s 2 Antibody, Alpha-NAC Antibody, HSD48 Antibody, NAC-alpha Antibody, NACA1 Antibody

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites).

Immunohistochemistry

Immunohistochemistry
Anti-NACA antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2470 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/10/2016

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