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Anti-MYL2 Antibody (clone AT3B2) IHC-plus™ LS-B3584

Note: This antibody replaces LS-C103487
See our new MYL2 ELISA Kits
Human MYL2 ELISA Kit - LS-F5141
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

MYL2 Antibody LS-B3584 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-MYL2 antibody IHC of human skeletal muscle.
Western blot
Western analysis of rat heart extracts

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Mouse Monoclonal [clone AT3B2] (IgG2b,k) to Human MYL2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human MYL2
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [AT3B2]
Protein G purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (1:1000 - 1:2000), ELISA

Specificity and Use

MYL2 antibody was raised against recombinant human MYL2 (1-166aa) purified from E. coli
human MYL2 (1-166aa)
Immunohistochemistry: LS-B3584 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3584 was determined to be 5 ug/ml. Western Blot: Extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About MYL2

P10916 NM_000432 NP_000423.2

MYL2 Antibody, MLC2 Antibody, Myosin light chain 2 Antibody, MLC-2 Antibody, RLC of myosin Antibody, CMH10 Antibody, MLC-2v Antibody

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Immunohistochemistry

Immunohistochemistry
Anti-MYL2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3584 concentration 5 ug/ml.

Western blot

Western blot
Western analysis of rat heart extracts

Requested From: United States
Date Requested: 8/30/2015

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