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Anti-MYL2 Antibody (clone AT3B2) IHC-plus™ LS-B3584

Note: This antibody replaces LS-C103487
Catalog Size Price
LS-B3584-50 50 µl (1 mg/ml) Unavailable

Related Products

14 MYL2 Antibodies

Most Popular MYL2 Antibodies

Anti-MYL2 Antibody (clone AT3B2) IHC-plus™ LS-B3584
Mouse Monoclonal [clone AT3B2] (IgG2b,k) to Human MYL2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MYL2 Antibody (clone 7C9) IHC-plus™ LS-B8057
Mouse Monoclonal [clone 7C9] (IgG1) to Human MYL2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MYL2 Antibody (clone 3B9-B4) LS-C197730
Mouse Monoclonal [clone 3B9-B4] (IgG1,k) to Human MYL2
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunofluorescence Image
Anti-MYL2 Antibody (aa2-159, FITC) LS-C302771
Rabbit Polyclonal (IgG) to Human MYL2
Human
Western blot
FITC Conjugated
Western blot Image
Anti-MYL2 Antibody LS-C412498
Rabbit Polyclonal to Human MYL2
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated

100% Guaranteed
Mouse Monoclonal [clone AT3B2] (IgG2b,k) to Human MYL2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human MYL2
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [AT3B2]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA

Specificity and Use

MYL2 antibody was raised against recombinant human MYL2 (1-166aa) purified from E. coli
human MYL2 (1-166aa)
Immunohistochemistry: LS-B3584 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3584 was determined to be 5 ug/ml. Western Blot: Extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About MYL2

P10916 NM_000432 NP_000423.2

MYL2 Antibody, MLC2 Antibody, Myosin light chain 2 Antibody, MLC-2 Antibody, RLC of myosin Antibody, CMH10 Antibody, MLC-2v Antibody

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Immunohistochemistry

Immunohistochemistry
Anti-MYL2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3584 concentration 5 ug/ml.

Western blot

Western blot
Western analysis of rat heart extracts

Requested From: 
Date Requested: 12/12/2017

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