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Anti-Myc Tag Antibody LS-C51564

Ordering

Wt. Vol. Conc. Price
40 µg - 0.5 mg/ml $215
100 µg - - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a)
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Myc Tag
Mouse
IgG2a Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

Myc Tag antibody was raised against a synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Mouse, Rat (100%); Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (90%); Horse, Pufferfish, Paramecium, Salpingoeca (80%).
C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc genes in human cancers, such as Hela whole cell lysates

Packaging

Lyophilized from PBS, pH 7.4, 0.02% sodium azide.
deionized water
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Myc Tag


Immunocytochemistry

Immunocytochemistry
Immunofluorescence of 293 cells transfected with c-Myc tag protein using THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) LS-C51564

Western blot

Western blot
Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse with series of loading amounts and antibody concentration. A: 0.2 ug/ml c-Myc Tag Antibody (equivalent to 1:5000 diluted from 1.0 mg/ml) B: 0.1 ug/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml) C: 0.05 ug/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml) The signal was developed with IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG

Western blot

Western blot
Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse with series of loading amounts and antibody concentration. A: 0.2 ug/ml c-Myc Tag Antibody (equivalent to 1:5000 diluted from 1.0 mg/ml) B: 0.1 ug/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml) C: 0.05 ug/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml) The signal was developed with IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG

Western blot

Western blot
20 ug Multiple Tag Cell Lysate (M0100) was used for the IP using THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) LS-C51564 and half of th IP was loaded on the gel. The Western blot was performed by One-Step IP-Western Kit (Mouse) (L00232)

Western blot

Western blot
Western blot discovers working concentration comparison of THETM c-Myc Tag Antibody, mAb, Mouse with Mouse Anti-c-Myc mAb (9E10), showing LS-C51564 posses excellent performance in WB assay with surprising antibody dilution. Both c-Myc antibodies starting dilution are from 1:1000 (equivalent to 1.0 ug/ml) in assay. The signal was developed with IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG.
 

Requested From: United States
Date Requested: 2/21/2017

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