Myc Tag

Mouse

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Percent identity by BLAST analysis: Mouse (100%); Human, Chimpanzee, Gorilla, Rat, Amoeba (91%); Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).

aa410-420

Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

• Western blot
• Immunoprecipitation

Suitable for use in Western Blot. Western Blot Analysis: 0.5-2.0 ug/ml detects Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag. Lysates from NIH/3T3 cells transfected with either empty vector, PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLLRKRGST, or PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLNGST were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Myc Tag (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Myc-tagged PP2A A subunit (~63kD). Longer exposure demonstrating weak detection by anti-Myc Tag of the Myc-tagged protein containing the epitope tag sequence MEQKLISEEDLNGST. Immunoprecipitation: Reported that this antibody immunoprecipitates Myc-tagged protein from transfected cells.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

For research use only. Intended for use by laboratory professionals.

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