Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Myc Tag antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Percent identity by BLAST analysis: Mouse (100%); Human, Chimpanzee, Gorilla, Rat, Amoeba (91%); Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.
Suitable for use in Western Blot. Western Blot Analysis: 0.5-2.0 ug/ml detects Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag. Lysates from NIH/3T3 cells transfected with either empty vector, PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLLRKRGST, or PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLNGST were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Myc Tag (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Myc-tagged PP2A A subunit (~63kD). Longer exposure demonstrating weak detection by anti-Myc Tag of the Myc-tagged protein containing the epitope tag sequence MEQKLISEEDLNGST. Immunoprecipitation: Reported that this antibody immunoprecipitates Myc-tagged protein from transfected cells.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.