(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Paraffin (1 - 2 µg/ml)
- Western blot (1:30 - 1:100)
- ELISA (1:100 - 1:1000)
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including antigen retrieval solutions, blocking agents
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MTA1 antibody was raised against synthetic peptide derived from the C-terminal region of the human MTA1 protein.
Specific for the C-terminal region of the human MTA1 protein. On Western blots, it identifies doublets at ~80kD. A band of unknown origin is observed at ~150kD. Reactivity has been confirmed with human T47D cell lysates and rat testis homogenates by Western blotting, and with formalin-fixed, paraffin-embedded human breast cancer tissues by immunohistochemistry. Based on amino acid sequence homology, reactivity with mouse is also expected.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:100-1:1000. Western Blot: 1:30-1:100. Identifies doublets at ~80kD and a band of unknown origin at ~150kD. Immunohistochemistry (Paraffin): 1:50-1:100. With formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining. Positive control: Human T47D cell lysates and rat testis homogenates (WB), human breast cancer tissues (IHC).
0.1% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
For research use only.