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Anti-MTA1 Antibody (C-Terminus) LS-C9003

Ordering

Wt. Vol. Conc. Price
- 1000 µl - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Mouse Monoclonal (IgG2b,k) to Human MTA1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human MTA1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal
Tissue culture supernatant

Applications

IHC - Paraffin (1 - 2 µg/ml), Western blot (1:30 - 1:100), ELISA (1:100 - 1:1000)

Specificity and Use

MTA1 antibody was raised against synthetic peptide derived from the C-terminal region of the human MTA1 protein.
C-Terminus
Specific for the C-terminal region of the human MTA1 protein. On Western blots, it identifies doublets at ~80kD. A band of unknown origin is observed at ~150kD. Reactivity has been confirmed with human T47D cell lysates and rat testis homogenates by Western blotting, and with formalin-fixed, paraffin-embedded human breast cancer tissues by immunohistochemistry. Based on amino acid sequence homology, reactivity with mouse is also expected.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:100-1:1000. Western Blot: 1:30-1:100. Identifies doublets at ~80kD and a band of unknown origin at ~150kD. Immunohistochemistry (Paraffin): 1:50-1:100. With formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining. Positive control: Human T47D cell lysates and rat testis homogenates (WB), human breast cancer tissues (IHC).

Packaging

0.1% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About MTA1

Q13330 NM_004689 NP_004680.2

MTA1 Antibody, Metastasis associated 1 Antibody

Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator. As a part of the histone-deacetylase multiprotein complex (NuRD), regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA. In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A.

Requested From: United States
Date Requested: 10/1/2016

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