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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MSX2 / MSH Antibody (aa1‑77) LS‑C9106
MSH antibody LS-C9106 is an unconjugated mouse monoclonal antibody to MSH (MSX2) from human and rat. Validated for IHC and WB.
Antibody
Catalog
Size
Price
LS-C9106-100
100 µg
$475

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Immunohistochemistry image at a dilution of 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

MSH antibody LS-C9106 is an unconjugated mouse monoclonal antibody to MSH (MSX2) from human and rat. Validated for IHC and WB.

Specifications

Target
Human MSX2 / MSH
Synonyms
MSX2, CRS2, HOX8, Homeobox protein Hox-8, Homeobox protein MSX-2, Msh homeo box 2, Parietal foramina 1, PFM1, MSH, Msh homeobox 2, Msh homeobox homolog 2, PFM, FPP
Host
Mouse
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC (1 - 10 µg/ml)
  • Western blot (1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MSX2 / MSH antibody was raised against recombinant human Msx2 protein (corresponding to the N-terminal region, aa 1-77).
Epitope
aa1-77
Specificity
Recognizes an ~30kD protein, corresponding to the apparent molecular mass of human Msx2 at ~30kD. Species cross-reactivity: rat.
Usage
Suitable for use in Western Blot and Immunohistochemistry. Western Blot (ECL): 1 ug/ml. Immunohistochemistry: 1-10 ug/ml.
Presentation
Lyophilized
Reconstitution
100 µl deionized water
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About MSX2 / MSH
Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter. P35548 NM_002449 NP_002440.2


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Requested From: United States
Date Requested: 10/20/2018

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