Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - 4% paraformaldehyde, ICC, Immunofluorescence, Western blot
Human MSI1 / Musashi 1
Human, Monkey, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
IHC - 4% paraformaldehyde (1:150), ICC (1:100), Immunofluorescence, Western blot (1:500)
Specificity and Use
MSI1 / Musashi 1 antibody was raised against reacts with residues 5-21 [APQPGLASPDSPHDPCK] of the human, mouse and rat Musashi 1 protein. Percent identity by BLAST analysis: Human, Marmoset, Mouse, Rat, Bovine, Dog (100%); Xenopus (82%).
Musashi 1 - Neuronal Stem Cell Marker.
Affinity purified antiserum, 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Musashi-positive cells stained with Musashi antibody (1:150 dilution) in the ventricular zone of E14.5 mouse medulla. Image taken at 200x magnification. The sections were 4% PFA fixed, paraffin-embedded and cut at 5 microns.
Image of staining with Musashi polyclonal antibody (1:100 dilution) shows Musashi (green) staining on neural rosettes (human). Nuclei are counterstained blue (DAPI). Cells were fixed with 4% PFA. No antigen retrieval was done.
Western blot of Musashi-1 with Musashi-1 antibody at 1:500 dilution. 10 ug of rat neural stem cell lysate (lane 1) and normal human brain lysate (lane 2)
Requested From: United States
Date Requested: 9/26/2016