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Anti-MSI1 / Musashi 1 Antibody (aa5-21) LS-C2716

Note: This antibody replaces LS-C72915, LS-C9128, LS-C25672, LS-C72914

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Rabbit Polyclonal to Human MSI1 / Musashi 1
Human, Monkey, Mouse, Rat, Bovine, Dog
IHC - 4% paraformaldehyde, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human MSI1 / Musashi 1
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - 4% paraformaldehyde (1:150), ICC (1:100), Immunofluorescence, Western blot (1:500)

Specificity and Use

MSI1 / Musashi 1 antibody was raised against reacts with residues 5-21 [APQPGLASPDSPHDPCK] of the human, mouse and rat Musashi 1 protein. Percent identity by BLAST analysis: Human, Marmoset, Mouse, Rat, Bovine, Dog (100%); Xenopus (82%).
aa5-21
Musashi 1 - Neuronal Stem Cell Marker.

Packaging

Affinity purified antiserum, 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MSI1 / Musashi 1

O43347 NM_002442 NP_002433.1

MSI1 Antibody, Musashi homolog 1 (Drosophila) Antibody, Musashi RNA-binding protein 1 Antibody, Musashi (Drosophila) homolog 1 Antibody, Musashi1 Antibody, Musashi 1 Antibody, Musashi-1 Antibody

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.

Immunofluorescence

Immunofluorescence
Musashi-positive cells stained with Musashi antibody (1:150 dilution) in the ventricular zone of E14.5 mouse medulla. Image taken at 200x magnification. The sections were 4% PFA fixed, paraffin-embedded and cut at 5 microns.

Immunofluorescence

Immunofluorescence
Image of staining with Musashi polyclonal antibody (1:100 dilution) shows Musashi (green) staining on neural rosettes (human). Nuclei are counterstained blue (DAPI). Cells were fixed with 4% PFA. No antigen retrieval was done.

Western blot

Western blot
Western blot of Musashi-1 with Musashi-1 antibody at 1:500 dilution. 10 ug of rat neural stem cell lysate (lane 1) and normal human brain lysate (lane 2)
 

Requested From: United States
Date Requested: 9/26/2016

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