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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MSH4 Antibody (aa863‑912) LS‑C107500
MSH4 antibody LS-C107500 is an unconjugated rabbit polyclonal antibody to MSH4 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C107500-50
50 µg
Unavailable

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Western blot Image
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Product Description

MSH4 antibody LS-C107500 is an unconjugated rabbit polyclonal antibody to MSH4 from human, mouse, rat and other species. Validated for WB.
About MSH4
MSH4 is a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. O15457 NM_002440 NP_002431.2

MSH4 Antibody, MutS (E. coli) homolog 4 Antibody, HMSH4 Antibody, MutS homolog 4 (E. coli) Antibody, MutS protein homolog 4 Antibody


Specifications

Target
Human MSH4
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Horse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
MSH4 antibody was raised against synthetic peptide located between aa863-912 of human ADARB2 (O15457, NP_002431). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Panda, Dog, Bovine, Guinea pig (100%); Marmoset, Bat, Horse, Zebra finch, Trout (92%); Rabbit, Opossum, Stickleback (85%); Chicken (84%); Mouse, Rat (78%).
Blocking Peptide
LS-E15214 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C107500. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa863-912
Specificity
Human MSH4
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MSH4 antibody LS-C107500 Western blot of Fetal Thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MSH4 antibody LS-C107500 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MSH4 antibody LS-C107500 Western blot of Fetal Thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MSH4 antibody LS-C107500 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MSH4 antibody LS-C107500 Western blot of Fetal Thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MSH4 antibody LS-C107500 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MSH4 antibody LS-C107500 Western blot of Fetal Thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MSH4 antibody LS-C107500 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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