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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MRGPRX3 / MRGX3 Antibody (aa244‑293) LS‑C135660
MRGX3 antibody LS-C135660 is an unconjugated rabbit polyclonal antibody to MRGX3 (MRGPRX3) from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C135660-50
50 µg
Unavailable

Popular MRGPRX3 / MRGX3 Products

Anti-MRGPRX3 / MRGX3 antibody IHC of human brain, cerebellum, Purkinje neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A5120 dilution 21 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin
Anti-MRGPRX3 / MRGX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin
Immunofluorescence of MCF7 cells, using MRGX3 Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-MRGPRX3 / MRGX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin

Product Description

MRGX3 antibody LS-C135660 is an unconjugated rabbit polyclonal antibody to MRGX3 (MRGPRX3) from human and monkey. Validated for WB.
About MRGPRX3 / MRGX3
Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins. Q96LB0 NM_054031 NP_473372.3

MRGPRX3 Antibody, MAS-related GPR, member X3 Antibody, Hdrr1 Antibody, HDRR2 Antibody, MRGX3 Antibody, SNSR1 Antibody, SNSR2 Antibody, Mas-related GPCR member X3 Antibody


Specifications

Target
Human MRGPRX3 / MRGX3
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
MRGPRX3 / MRGX3 antibody was raised against synthetic peptide located between aa244-293 of human MRGPRX3 (Q96LA9, NP_473372). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).
Blocking Peptide
LS-E21935 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135660. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa244-293
Specificity
Human MRGPRX3 / MRGX3
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MRGPRX3 / MRGX3 antibody LS-C135660 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MRGPRX3 / MRGX3 antibody LS-C135660 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MRGPRX3 / MRGX3 antibody LS-C135660 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MRGPRX3 / MRGX3 antibody LS-C135660 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MRGPRX3 / MRGX3 antibody LS-C135660 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MRGPRX3 / MRGX3 antibody LS-C135660 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MRGPRX3 / MRGX3 antibody LS-C135660 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MRGPRX3 / MRGX3 antibody LS-C135660 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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