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Anti-Mouse Hepatitis Virus NSP9 Antibody (clone 2C6.H1) LS-C59526

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Wt. Vol. Conc. Price
100 µg - 2.1 mg/ml $405
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Most Popular Mouse Hepatitis Virus NSP9 Antibodies

Anti-Mouse Hepatitis Virus NSP9 Antibody (clone 2C6.H1) LS-C59526
Mouse Monoclonal [clone 2C6.H1] to Mouse Hepatitis Virus NSP9
Mouse Hepatitis Virus
Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image

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Mouse Monoclonal [clone 2C6.H1] to Mouse Hepatitis Virus NSP9
Mouse Hepatitis Virus
Immunofluorescence, Western blot
Unconjugated

Details

Mouse Hepatitis Virus NSP9
Mouse
Mouse Hepatitis Virus (tested or 100% immunogen sequence identity)
Monoclonal [2C6.H1]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (1:1000)
  • Western blot (1:1000)

Specificity and Use

E. coli derived full- length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
the MHV-A59 nsp9 protein. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologs from other sources has not been tested
This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kD in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHVA59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Mouse Hepatitis Virus NSP9


Immunofluorescence

Immunofluorescence
Anti-MHV-A59 nsp9 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using anti-MHV-A59 nsp9 antibody, 6-h post infection in mouse L cells. Cells were fixed in 3% para-formaldehyde. For detection Cy2 conjugated Goat-anti-Mouse IgG MX10 ( was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.

Western blot

Western blot
Anti-MHV-A59 nsp9 Antibody - Western Blot. Western blotting using anti-MHV-A59 nsp9 antibody to detect protein in various lysates, 6h post MHV infection. Lane 1 shows no cross-reactivity with SARS-CoV-infected Vero cells. Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3. Negative controls (lanes 2 and 4) show no staining. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.

Requested From: United States
Date Requested: 12/10/2016

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