Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 2C6.H1] to Mouse Hepatitis Virus NSP9
Mouse Hepatitis Virus
Immunofluorescence, Western blot
Mouse Hepatitis Virus NSP9
Mouse Hepatitis Virus (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1:1000)
Specificity and Use
E. coli derived full- length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
the MHV-A59 nsp9 protein. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologs from other sources has not been tested
This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kD in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHVA59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Anti-MHV-A59 nsp9 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using anti-MHV-A59 nsp9 antibody, 6-h post infection in mouse L cells. Cells were fixed in 3% para-formaldehyde. For detection Cy2 conjugated Goat-anti-Mouse IgG MX10 ( was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
Anti-MHV-A59 nsp9 Antibody - Western Blot. Western blotting using anti-MHV-A59 nsp9 antibody to detect protein in various lysates, 6h post MHV infection. Lane 1 shows no cross-reactivity with SARS-CoV-infected Vero cells. Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3. Negative controls (lanes 2 and 4) show no staining. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.