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Anti-MMP13 Antibody (aa20-471, clone 87512) LS-C150004

Ordering

Wt. Vol. Conc. Price
500 µg - - $385
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MMP13 Antibodies

Anti-MMP13 Antibody (N-Terminus) IHC-plus™ LS-A1552
Rabbit Polyclonal to Human MMP13
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-MMP13 Antibody (Internal) IHC-plus™ LS-A1553
Rabbit Polyclonal to Human MMP13
Human, Monkey, Horse, Pig, Rabbit
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-MMP13 Antibody IHC-plus™ LS-B3168
Rabbit Polyclonal to Rat MMP13
Rat, Human, Mouse, Bovine, Horse, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 87512] (IgG1) to Human MMP13
Human
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human MMP13
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [87512]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (8 - 25 µg/ml)
  • ICC (8 - 25 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation (25 µg/ml)

Specificity and Use

MMP13 antibody was raised against chinese hamster ovary cell line CHO-derived recombinant human MMP13 Leu20-Cys471 Accession # P45452.
aa20-471
Detects human MMP13 in direct ELISAs and Western blots. Detects both pro and active forms of human MMP13 in Western blots. In direct.
Western Blot, 1 µg/ml, Recombinant Human MMP-13 Western Blot Standard. Immunocytochemistry, 8-25 µg/ml. Immunohistochemistry, 8-25 µg/ml. Immunoprecipitation, 25 µg/ml, Conditioned cell culture medium spiked with Recombinant Human MMP-13.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Reconstitute at 0.5 mg/ml in sterile PBS
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About MMP13

P45452 NM_002427 NP_002418.1

MMP13 Antibody, Collagenase 3 Antibody, Matrix metalloproteinase-13 Antibody, Interstitial collagenase Antibody, MANDP1 Antibody, MMP-13 Antibody, CLG3 Antibody

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF.

Immunohistochemistry

Immunohistochemistry
MMP-13 in Human Ovarian Cancer Tissue. MMP-13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 8 ug/ml Mouse Anti-Human MMP-13 Monoclonal Antibody overnight at 4°C. Tissue was stained with Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).

Immunocytochemistry

Immunocytochemistry
MMP-1 3 in MDA-MB-231 Human Cell Line. MMP-13 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Human MMP-13 Monoclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Requested From: United States
Date Requested: 12/10/2016

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