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Anti-MLX / TCFL4 Antibody (aa197-298) LS-C72745

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $635
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MLX / TCFL4 Antibodies

Anti-MLX / TCFL4 Antibody (aa217-231) IHC-plus™ LS-B6494
Goat Polyclonal to Human MLX / TCFL4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MLX / TCFL4 Antibody (Internal, AP) LS-C206741
Rabbit Polyclonal (IgG) to Human MLX / TCFL4
Human
Western blot, ELISA
AP Conjugated
Anti-MLX / TCFL4 Antibody (Internal, Biotin) LS-C206743
Rabbit Polyclonal (IgG) to Human MLX / TCFL4
Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human MLX / TCFL4
Human
ICC, Western blot, ELISA
Unconjugated

Details

Human MLX / TCFL4
Goat
Human (tested or 100% immunogen sequence identity)
Mouse, Dog (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

MLX / TCFL4 antibody was raised against e. coli-derived recombinant human rhMLX (aa 197-298).
aa197-298
Recognizes human MLX. Species sequence homology: 99% identical to mouse and canine MLX.
Suitable for use in Western blot, Immunocytochemistry and ELISA. Western blot: 0.1-0.2 ug/ml. The detection limit is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml will detect human MLX in serum stimulated W138 human fetal lung fibroblasts. Fix cells with PBS containing 4% paraformaldehyde for 20 minutes at RT and block with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml. The detection limit is ~0.3 ng/well.

Packaging

Lyophilized powder from PBS, 5% trehalose. Reconstitute, 0.5 ml PBS to a concentration of 0.2 mg/ml.
100 µl PBS
For research use only.

About MLX / TCFL4

Q9UH92 NM_170607 NP_733752.1

MLX Antibody, BHLHd13 Antibody, BigMax protein Antibody, MXD7 Antibody, MAD7 Antibody, Max-like bHLHZip protein Antibody, TCFL4 Antibody, MLX, MAX dimerization protein Antibody, Protein BigMax Antibody, Max-like protein X Antibody, Transcription factor-like 4 Antibody

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.

Requested From: United States
Date Requested: 12/7/2016

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