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Anti-MLX / TCFL4 Antibody (aa197-298) LS-C72745

Catalog Size Price
LS-C72745-100 100 µg Unavailable

Related Products

44 MLX / TCFL4 Antibodies

Most Popular MLX / TCFL4 Antibodies

Anti-MLX / TCFL4 Antibody (aa217-231) IHC-plus™ LS-B6494
Goat Polyclonal to Human MLX / TCFL4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
IHC, IHC - Paraffin, ELISA
Unconjugated
Anti-MLX antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6494 concentration 2.5 ug/ml.
Anti-MLX / TCFL4 Antibody (aa125-151) LS-C164223
Rabbit Polyclonal to Human MLX / TCFL4
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with MLX Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Anti-MLX / TCFL4 Antibody (Internal) LS-C352918
Rabbit Polyclonal to Human MLX / TCFL4
Human, Mouse, Rat, Bovine
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of TCFL4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-MLX / TCFL4 Antibody (aa80-160) LS-C384573
Rabbit Polyclonal (IgG) to Human MLX / TCFL4
Human, Mouse, Rat
IHC, Western blot, ELISA
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Anti-MLX / TCFL4 Antibody LS-C666797
Rabbit Polyclonal (IgG) to Human MLX / TCFL4
Human
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed
Goat Polyclonal (IgG) to Human MLX / TCFL4
Human
ICC, Western blot, ELISA
Unconjugated

Details

Human MLX / TCFL4
Goat
Human (tested or 100% immunogen sequence identity)
Mouse, Dog (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

MLX / TCFL4 antibody was raised against e. coli-derived recombinant human rhMLX (aa 197-298).
aa197-298
Recognizes human MLX. Species sequence homology: 99% identical to mouse and canine MLX.
Suitable for use in Western blot, Immunocytochemistry and ELISA. Western blot: 0.1-0.2 ug/ml. The detection limit is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml will detect human MLX in serum stimulated W138 human fetal lung fibroblasts. Fix cells with PBS containing 4% paraformaldehyde for 20 minutes at RT and block with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml. The detection limit is ~0.3 ng/well.

Packaging

Lyophilized powder from PBS, 5% trehalose. Reconstitute, 0.5 ml PBS to a concentration of 0.2 mg/ml.
100 µl PBS
For research use only.

About MLX / TCFL4

Q9UH92 NM_170607 NP_733752.1

MLX Antibody, BHLHd13 Antibody, BigMax protein Antibody, MXD7 Antibody, MAD7 Antibody, Max-like bHLHZip protein Antibody, TCFL4 Antibody, MLX, MAX dimerization protein Antibody, Protein BigMax Antibody, Max-like protein X Antibody, Transcription factor-like 4 Antibody

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.

Requested From: 
Date Requested: 4/25/2018

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