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Anti-MLC2 / MYL9 Antibody (C-Terminus) IHC-plus™ LS-B5814

Note: This antibody replaces LS-C81207

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MLC2 / MYL9 Antibodies

Anti-MLC2 / MYL9 Antibody (aa12-27) IHC-plus™ LS-B38
Rabbit Polyclonal to Human MLC2 / MYL9
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MLC2 / MYL9 Antibody (C-Terminus) IHC-plus™ LS-B5814
Rabbit Polyclonal (IgG) to Human MLC2 / MYL9
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MLC2 / MYL9 Antibody LS-C332334
Rabbit Polyclonal (IgG) to Human MLC2 / MYL9
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal (IgG) to Human MLC2 / MYL9
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human MLC2 / MYL9
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

MLC2 / MYL9 antibody was raised against synthetic peptide from C-Terminus of human MYL9 (P24844, NP_006088). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Frog (100%); Seabass, Catfish, Salmon, Smelt, Sablefish, Stickleback, Pike, Zebrafish (92%); Trout, Pufferfish (85%).
LS-E17473 - Lyophilized - 100 µg - $145.00
C-Terminus
Human MYL9
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (2)

1
Phos-tag-based analysis of myosin regulatory light chain phosphorylation in human uterine myocytes. Aguilar HN, Tracey CN, Tsang SC, McGinnis JM, Mitchell BF. PloS one. 2011 6:e20903. [Full Text Article] [PubMed:21695279] [PMC:PMC3111472]
2
Rho-kinase mediates diphosphorylation of myosin regulatory light chain in cultured uterine, but not vascular smooth muscle cells. Aguilar HN, Tracey CN, Zielnik B, Mitchell BF. Journal of cellular and molecular medicine. 2012 Dec;16:2978-89. [Full Text Article] [PubMed:22947248]

About MLC2 / MYL9

P24844 NM_006097 NP_006088.2

MYL9 Antibody, 20 kDa myosin light chain Antibody, LC20 Antibody, MLC-2C Antibody, MLC2 Antibody, Myosin RLC Antibody, MRLC1 Antibody, MYRL2 Antibody

Myosin, the major component of muscle filaments, is a long asymmetric molecule containing a globular head and a long tail. It is composed of two heavy chains and four light chains. Phosphorylation of myosin light chain in response to increased calcium levels leads to contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.

Immunohistochemistry

Immunohistochemistry
Anti-MYL9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5814 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/11/2016

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