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Anti-MKRN1 Antibody (aa105-118) LS-C154849

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MKRN1 Antibodies

Anti-MKRN1 Antibody (aa184-213, Biotin) LS-C237674
Rabbit Polyclonal (IgG) to Human MKRN1
Human
Western blot, ELISA
Biotin Conjugated
Anti-MKRN1 Antibody (aa184-213, PE) LS-C237677
Rabbit Polyclonal (IgG) to Human MKRN1
Human
Western blot, ELISA
PE Conjugated
Anti-MKRN1 Antibody (aa184-213, Azide-free, HRP) LS-C248825
Rabbit Polyclonal (IgG) to Human MKRN1
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human MKRN1
Human, Monkey, Bat, Bovine
Western blot, ELISA
Unconjugated

Details

Human MKRN1
Goat
Human, Monkey, Bat, Bovine (tested or 100% immunogen sequence identity)
Mouse, Rat, Dog, Hamster, Horse (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:64000)

Specificity and Use

MKRN1 antibody was raised against synthetic peptide C-RYEHSKPLKQEEAT from an internal region of human MKRN1 (NP_038474.2; NP_001138597.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Bovine, Bat, Elephant (100%); Orangutan, Gibbon, Mouse, Rat, Dog, Hamster, Panda, Horse, Porcine (93%).
LS-E27676 - Lyophilized - 100 µg - $145.00
aa105-118
Human MKRN1. This antibody is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1).
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged), a band of approx. 60kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.3-1 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About MKRN1

Q9UHC7 NM_013446 NP_038474.2

MKRN1 Antibody, Makorin ring finger protein 1 Antibody, RNF61 Antibody, RING finger protein 61 Antibody

MKRN1 is a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription.

Western blot

Western blot
MKRN1 antibody HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with (0.3 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/9/2016

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