Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
MKI67 / Ki67 antibody was raised against nuclear protein preparation from the human cell line U937.50.
Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Confined to the nuclei of proliferating cells and no cross-reactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Western Blot: 1:500. Detected cell-cycle-associated protein of 345kD and 395kD on 10 ug of A431 lysates. Immunocytochemistry: 5-10 ug/ml. Immunohistochemistry: 5-10 ug/ml. Positive control: Tonsil tissue, A431 Cell Lysate.
PBS, pH 7.6, 0.09% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.