Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone Ki-S5] (IgG1) to Human MKI67 / Ki67
IHC - Paraffin, ICC, Western blot, Flow Cytometry
Human MKI67 / Ki67
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [Ki-S5]
Protein A purified
IHC - Paraffin (10 µg/ml)
ICC (5 - 10 µg/ml)
Western blot (1:500)
Specificity and Use
MKI67 / Ki67 antibody was raised against nuclear protein preparation from the human cell line U937.50.
Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Confined to the nuclei of proliferating cells and no cross-reactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Western Blot: 1:500. Detected cell-cycle-associated protein of 345kD and 395kD on 10 ug of A431 lysates. Immunocytochemistry: 5-10 ug/ml. Immunohistochemistry: 5-10 ug/ml. Positive control: Tonsil tissue, A431 Cell Lysate.
PBS, pH 7.6, 0.09% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.