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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MITF Antibody (Gly174) LS‑C176425
MITF antibody LS-C176425 is an unconjugated rabbit polyclonal antibody to MITF from human and mouse. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C176425-100
100 µl
Unavailable

Popular MITF Products

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bat, Bovine, Dog, Goat, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Zebrafish
Applications: Western blot
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Confocal immunofluorescence of MITF Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Western blot of extracts from COS7 cells, using MITF (Phospho-Ser180/73) Antibody. The lane on the right was incubated with synthetic peptide.
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Antibody
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

MITF antibody LS-C176425 is an unconjugated rabbit polyclonal antibody to MITF from human and mouse. Validated for IF, IHC and WB.
About MITF
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). O75030 NM_000248 NP_000239.1

MITF Antibody, BHLHe32 Antibody, CMM8 Antibody, MI Antibody, Waardenburg syndrome, type 2A Antibody, WS2 Antibody, WS2A Antibody


Specifications

Target
Human MITF
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MITF antibody was raised against synthetic peptide around Gly174 of human MITF
Epitope
Gly174
Specificity
Human MITF
Presentation
PBS, pH 7.2, 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of MITF (G174) pAb in extracts from COS7 cells.
Western blot of MITF (G174) pAb in extracts from COS7 cells.

Western blot

Western blot of MITF (G174) pAb in extracts from COS7 cells.
Western blot of MITF (G174) pAb in extracts from COS7 cells.

Western blot

Western blot of MITF (G174) pAb in extracts from COS7 cells.
Western blot of MITF (G174) pAb in extracts from COS7 cells.

Western blot

Western blot of MITF (G174) pAb in extracts from COS7 cells.
Western blot of MITF (G174) pAb in extracts from COS7 cells.


Requested From: 
Date Requested: 6/22/2018

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