Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone OX-3] (IgG1) to Rat MHC Class II RT1.Bu
IHC - Paraffin, IHC - Frozen, Flow Cytometry
Rat MHC Class II RT1.Bu
Rat, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [OX-3]
Ion exchange chromatography
IHC - Paraffin
IHC - Frozen
Flow Cytometry (1:100)
Specificity and Use
Rat thymocyte membrane glycoproteins.
Recognizes a polymorphic determinant of rat Ia antigen (RT1. Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. Is useful for distinguishing Ia positive cells from different rat strains, e. g. for recognizing cells of donor origin in bone marrow reconstituted radiation chimaeras. MRC OX-3 Reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells. This product is routinely tested in flow cytometry on Lewis rat splenocytes.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.