Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone P7/7] (IgG2b) to Rat MHC Class II I-Ab
IHC - Frozen, Flow Cytometry
Rat MHC Class II I-Ab
Rat, Mouse (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [P7/7]
IHC - Frozen
Flow Cytometry (1:1 - 1:4)
Specificity and Use
Cells from R8 strain rat.
Recognizes the rat RT1Bu/Du MHC class II antigen and is cross reactive with haplotypes c, d, l, and n. The antibody positively stains MHC class II molecules in rat strains including PVG, F334, LEW, and BN. Clone P7/7 also recognizes mouse MHC Class II, binding to a monomorphic epitope expressed by all mouse strains.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Tissue culture supernatant, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.