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Anti-MHC Class II Antibody (clone M5/114, Azide-free) LS-C204829


Wt. Vol. Conc. Price
100 µg - 1 mg/ml $245
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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Rat Monoclonal [clone M5/114] (IgG2b) to Mouse MHC Class II
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, Blocking, Functional Assay, Neutralization
Unconjugated, Azide-free


Mouse MHC Class II
Mouse (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [M5/114]
Protein G purified


  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • Blocking
  • Functional Assay
  • Neutralization

Specificity and Use

MHC Class II antibody was raised against activated C57BL/6 mouse spleen cells.
Monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Flow Cytometry: Recommended dilution - 2 ug/ml. Functional Application: Blocking of T cell proliferative responses.


PBS, pH 7.4, sterile filtered.
Store at 2-8°C. Do not freeze.
For research use only.

About MHC Class II

MHC (major histocompatibility complex) class II molecules are a family of molecules normally found only on antigen-presenting cells such as dendritic cells, mononuclear phagocytes, some endothelial cells, thymic epithelial cells, and B cells. The antigens presented by class II peptides are derived from extracellular proteins (not cytosolic as in class I); hence, the MHC class II-dependent pathway of antigen presentation is called the endocytic or exogenous pathway.

Requested From: United States
Date Requested: 10/27/2016

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